Human breast cancer cell lines MCF 7 and MCF 7 ADR cells were cultured in RPMI 1640 medium GIBCO, Grand Island, NY, MDA MB 231 cells were cultured in Leibovitz s L 15 medium GIBCO, Grand Island, NY, USA how to take priligy Although exogenous estrogen may be given either as part of combined estrogen- progestin therapy or in the form of combined oral contraceptives, sequential therapy with exogenous estrogen and progestin is most physiologic